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Image Search Results
Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Article Title: Age- and Sex-Associated Plasma Proteomic Changes in Growth Hormone Receptor Gene-Disrupted Mice
doi: 10.1093/gerona/glr212
Figure Lengend Snippet: 2-D gel image with mouse plasma proteins. All proteins have been identified with multiple isoforms numbered 1, 2, etc. Mw = molecular weight; pI = isoelectric point; APOA4 = apolipoprotein A-4; APOA1 = apolipoprotein A-1; APOE = apolipoprotein E; HBB = hemoglobin subunit beta; HP = haptoglobin; MBP-C = mannose-binding protein-C; RBP-4 = retinol-binding protein-4.
Article Snippet: Antibodies used included
Techniques: Clinical Proteomics, Molecular Weight, Binding Assay
Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Article Title: Age- and Sex-Associated Plasma Proteomic Changes in Growth Hormone Receptor Gene-Disrupted Mice
doi: 10.1093/gerona/glr212
Figure Lengend Snippet: Differential plasma proteins in GHR−/− mice compared with WT mice. (A) Cropped 2-D gel images showing APOA4 isoforms 2, 3, and 4 and APOE isoforms 1, 3, and 4. (B) 3-D view of intensities of APOA4 isoforms 2, 3, and 4 and APOE isoforms 1, 3, and 4. (C) Western blotting on APOA4, APOE, and HP in 24-month-old male mice. Equal protein loading was verified by Commassie blue staining of sodium dodecyl sulfate–polyacrylamide gel electrophoresis gels (not shown). (D) Quantitative real-time polymerase chain reaction analysis of MBP-C messenger RNA expression in the liver of 24-month-old male mice. Data were normalized relative to expression of three reference genes Actb, Hmbs, and Hprt using qBase Plus 2.0 program. APOE = apolipoprotein E; HP = haptoglobin; MBP-C = mannose-binding protein-C; WT = wild-type.
Article Snippet: Antibodies used included
Techniques: Clinical Proteomics, Western Blot, Staining, Polyacrylamide Gel Electrophoresis, Real-time Polymerase Chain Reaction, RNA Expression, Expressing, Binding Assay